Faith Laboratory in the Icahn School of Medicine at Mount Sinai; Reverse engineering the interactions between our diets, our gut microbes and microbiome, and our health

Facilities and Equipment

GMP anaerobic manufacturing

Anaerobic GMP manufacturing
We have a custom anaerobic Coy chamber dedicated for GMP manufacturing of live biotherapeutic products for phase Ib/IIa clinical trials.

gnotobiotics

Gnotobiotic Facility
The gnotobiotic facility in the Microbiome Translational Center has multiple breeding isolators, small experimental isolators, and positive pressure cages.

biotyper

MALDI biotyper
The Bruker Biotyper allows culture collections of bacteria to be identified and QC'd in real time. It is also used to verify strain identity in GMP manufacturing runs of live biotherapeutic products.

coy chamber quad size

High throughput culturing
We have a four person, extra-long, coy chamber with an Opentrons liquid handling robot for high throughput culture collection generation and propagation.

plate filler

Plate filler
Automatic non-contact liquid dispensing for very high throughput assays. Featuring a custom styrofoam raft so it can float away in the case of an extreme flood. Looks rough on the outside but still a top performer.

stomacher

Stomacher
Automated stomacher to facilitate generation of concentrated stool filtrates for making fecal transplants.

elisa plate washer

Plate Washer and Stacker
High throughput plate washer with robotic stacker for running lots of ELISAs

opentrons

Liquid handling robotics
Opentrons. We have one anaerobic and two in the main lab for high throughput culturing and assays.

stool smasher

Stool smasher
One-of-a-kind, custom-machined, steel device for pulverizing stool into usable sizes. Extremely durable and destined to outlive us all. (smashed banana for demonstration purposes only.

beat beater

Bead beater
Loud but effective. There's no hiding what you're doing when you're extracting DNA in the lab.

pcr machines

PCR fleet
Small army of PCR machines.

Resources

Strainer screen shot

Software for tracking tracking strains in metagenomes using the strain reference genome. On github.

Gnoto screen shot

Gnoto
Mobile app to monitor gnotobiotic mouse breeding. On github.

M3D screen shot

M3D
A database of uniformly normalized and annotated microbial Affymetrix gene expression profiles from Large-Scale Mapping and Validation of Escherichia coli Transcriptional Regulation from a Compendium of Expression Profiles

Simulation effector strain

Phenotype Effector Strain simulator
Simulate community size -vs- total effect size from Identifying Gut Microbe-Host Phenotype Relationships Using Combinatorial Communities in Gnotobiotic Mice.