Anaerobic GMP manufacturingWe have a custom anaerobic Coy chamber dedicated for GMP manufacturing of live biotherapeutic products for phase Ib/IIa clinical trials.
Gnotobiotic FacilityThe gnotobiotic facility in the Microbiome Translational Center has multiple breeding isolators, small experimental isolators, and positive pressure cages.
MALDI biotyperThe Bruker Biotyper allows culture collections of bacteria to be identified and QC'd in real time. It is also used to verify strain identity in GMP manufacturing runs of live biotherapeutic products.
High throughput culturingWe have a four person, extra-long, coy chamber with an Opentrons liquid handling robot for high throughput culture collection generation and propagation.
Plate fillerAutomatic non-contact liquid dispensing for very high throughput assays. Featuring a custom styrofoam raft so it can float away in the case of an extreme flood. Looks rough on the outside but still a top performer.
StomacherAutomated stomacher to facilitate generation of concentrated stool filtrates for making fecal transplants.
Plate Washer and StackerHigh throughput plate washer with robotic stacker for running lots of ELISAs
Liquid handling roboticsOpentrons. We have one anaerobic and two in the main lab for high throughput culturing and assays.
Stool smasherOne-of-a-kind, custom-machined, steel device for pulverizing stool into usable sizes. Extremely durable and destined to outlive us all. (smashed banana for demonstration purposes only.
Bead beaterLoud but effective. There's no hiding what you're doing when you're extracting DNA in the lab.
PCR fleetSmall army of PCR machines.
Software for tracking tracking strains in metagenomes using the strain reference genome. On github.
GnotoMobile app to monitor gnotobiotic mouse breeding. On github.
M3DA database of uniformly normalized and annotated microbial Affymetrix gene expression profiles from Large-Scale Mapping and Validation of Escherichia coli Transcriptional Regulation from a Compendium of Expression Profiles
Phenotype Effector Strain simulatorSimulate community size -vs- total effect size from Identifying Gut Microbe-Host Phenotype Relationships Using Combinatorial Communities in Gnotobiotic Mice.